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CONTENTSPreface to the seventh edition pagexiList of contributorsxiiiList of abbreviationsxv1 Basic principles1K. WILSON1.1 Biochemical and molecular biology studies 11.2 Units of measurement 31.3 Weak electrolytes 61.4 Quantitative biochemical measurements 161.5 Safety in the laboratory 351.6 Suggestions for further reading 372 Cell culture techniques38A.R. BAYDOUN2.1 Introduction 382.2 The cell culture laboratory and equipment 392.3 Safety considerations in cell culture 432.4 Aseptic techniques and good cell culture practice 442.5 Types of animal cell, characteristics and maintenance in culture 492.6 Stem cell culture 612.7 Bacterial cell culture 682.8 Potential use of cell cultures 712.9 Suggestions for further reading 723 Centrifugation73K. OHLENDIECK3.1 Introduction 733.2 Basic principles of sedimentation 743.3 Types, care and safety aspects of centrifuges 793.4 Preparative centrifugation 863.5 Analytical centrifugation 953.6 Suggestions for further reading 99v