Page 23 - MNU PM502 Ph. Micreo practical
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Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)
Autoclave Pressure, Sterilization Temperature, and Time:
Temperature (℃) Pressure(psi) Time
115℃ 10 psi 30 minutes
121℃ 15 psi 15 minutes
132℃ 27 psi 10 minutes
134℃ 30 psi 2 minutes
The most commonly used standard temperature-time cycles is 121°C for 15
minutes.
Uses of Autoclave
Autoclave is particularly useful for media-containing water that cannot be
sterilized by dry heat. It is the method of choice for sterilizing the following:
10
