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Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025
Liver disease Renal disease Bone marrow
Protein Normal value Tumor
Albumin 3.5-5.5 g/dl ↓ ↓ Normal
Total proteins 6-8 g/dl ↓ ↓ ↑
Globulins 1.8-3.3 g/dl Normal ↓ ↑↑↑
A/G ratio 2/1 1/2 2/1 1/2
Practical work
Assessment of plasma total proteins:
Method: colorimetric method.
Principle: Proteins react with copper ions (II) to produce a bluish-violet
color compound in an alkaline medium.
++
Protein + Cu Colored Complex
Alkal
i
The color intensity is proportional to the concentration of total proteins
present in the sample.
Procedure: as stated in the lab.
Calculations:
Concentration of plasma total protein =
Conclusion:
The patient has
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