Page 80 - Practical Handout bio 2 mnu 2025_Neat
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Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025
Assessment of Albumin:
Method: colorimetric method.
Principle: Albumin is bound by the BCG dye to produce an increase in
the bluish-green color in a pH 3.8 acid medium.
The color increase is proportional to the concentration of albumin present
in the sample. Albumin determination is useful in the diagnosis of hepatic
and renal pathologies.
Procedure: as stated in the lab.
Calculations:
Concentration of plasma albumin =
Conclusion:
The patient has ………………………………………………………
Plasma Proteins
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