Page 75 - Practical Handout bio 2 mnu 2025_Neat
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Level two – Semester 4 - PharmD Biochemistry-II PB-403 (Practical course) 2024/2025
Liver function testing
1- Aminotransferases
Enzymes that catalyze the transfer of amino group from amino acid to α-
keto acid → to form a new amino acid.
Aspartate Alanine aminotransferase
aminotransferase (AST) (ALT)
= Glutamate oxaloacetate = Glutamate pyruvate
transaminase (GOT) transaminase (GPT)
Normal
< 41 U/L < 45 U/L
value
They are present intracellularly (inside liver cells) → so
the presence of elevated levels of them in the blood
indicates destruction (necrosis) of liver cells that contain
them.
Diagnostic
value
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