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Level two – Semester 4 - PharmD      Biochemistry-II PB-403 (Practical course)     2024/2025


                     Urine





                                     Test               Patient          Reference range




                              Urine osmolality 250 mOsm/kg (300-900 mOsm/kg)
                                Urine sodium         26 mmol/day      (40-220 mmol/day)






                          1)  What is the most likely diagnosis?
                   -----------------------------------------------------------------------------------------


                   -


                       2)  What is the further test that should be performed to confirm
                          the diagnosis? What are the expected results of the performed


                          tests?
                   ---------------------------------------------------------------------------------


                    ---------


                       3)  Explain the low urine osmolality in the previous case.

                   ---------------------------------------------------------------------------------

                    ---------


                                                    Practical work


                   Assessment of blood and urine glucose:


                   Method: colorimetric method.


                   Principle:


                   The  enzymatic  method  uses  Glucose  oxidase  (GOD)  to  catalyze  the

                   oxidation of glucose to hydrogen peroxide and gluconic acid.








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