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Original Article
General Vaccination and Hepatitis B Seyyed Amir Seyyedi, et al.

IR.UMSU.REC.1392.108). This descriptive study was results of other studies.
conducted on 72 dental students who were vaccinated The measures of central dispersion were reported for
against hepatitis B at birth. The sample size was calculated quantitative variables (mean and standard deviation), and the
according to a previous study with 95% confidence interval, frequency and percentage were calculated for qualitative
5% standard error rate and 47% prevalence of hepatitis B variables. Appropriate graphs and statistical tables were
2
vaccination in China. used to display the data as needed.
The participants were asked to mention the date of their last All statistical analyses were performed using IBM SPSS
hepatitis B vaccination, and participants who had been (IBM, Chicago, USA) version 21.0. Comparison of
vaccinated in the past 5 years were excluded. All vaccination efficacy between males and females was
participants had been vaccinated with inactivated-type conducted using the Chi-square test. P<0.05 was considered
vaccines of viral surface proteins. Five milliliters of statistically significant.
intravenous blood was taken from each student and sent to a
laboratory. In the laboratory, after separation of serum, HBs Results
antibody titer was measured by a Bind Mono kit (USA) by
ELISA. Then, the test results of each student were divided The present study was performed to evaluate the adequacy
into three groups based on the antibody titer obtained: of hepatitis B vaccination in 72 dental students in Urmia. Of
antibody level more than 100 IU/L, antibody level between the total participants in the study, 38 (52.7%) were males,
10-100 IU/L and antibody level below 10 IU/L. A positive and 34 (47.2%) were females.
response indicated safety, low positive response indicated The mean level of HBs antigen serum level was 25.035 ±
7
safety, and no response indicated no safety. 25.842 mlU/mL. Table 1 shows the frequency of HBs-Ab
The antibody titer evaluator was an expert, and the samples adequacy of the dental students. According to the findings, 7
were evaluated by an ELISA kit (Bind Mono, USA) with (9.7%) students had no immune response, 23 (31.9%) had
three repetitions, and the mean value was reported. The low safety, and 42 (58.3%) had good and acceptable safety.
ELISA kit is used to accurately evaluate antibodies There was no statistically significant difference between
worldwide, and therefore the results were comparable to the males and females in terms of antibody adequacy (P>0.05).

Table 1: Frequency of different modes of immunity against hepatitis B in dental students
Males Females
Variable Antibody Efficiency
Number Percentage Number Percentage
No safety 4 5.8 3 3.5
HBs-Ab Low safety 13 20.8 10 10.9
Good safety 22 31.2 20 27.8
Total 39 52.8 33 47.2

Discussion contagious agents on a regular basis and the risk of
infection will always be considerably high for them.
Hepatitis B is one of the most serious medical conditions This finding shows that all healthcare workers who are
among dental students. Dental students are permanently exposed to infectious agents need to be safe before entering
exposed to this condition due to exposure to patients and the workplace to protect their immune system against such
14
injured mucosal surfaces. Compulsory vaccination against infections. Izadpanah et al. examined the antibody titer
this virus has been carried out in Iran since 1993. Adequacy against HBV in nurses of Birjand University of Medical
of hepatitis B vaccination was assessed in 72 students. Of Sciences. The aim of their study was to determine the
the total participants in the study, 38 (52.7%) were males degree of immunization following complete hepatitis B
and 34 (47.2%) were females. vaccination in the nursing staff and the relationship
According to our findings, most of the students were between safety level and variables such as age, sex, body
immune to this virus, although about 32% of them showed mass index, workplace and time elapsed since the last dose
low immunity, with no significant difference between of vaccine. In their study, the antibody titer in 88.4% of
males and females, indicating the need for re-vaccination. the subjects was above 10 mlU/mL. The level of protective
Seven people (4 males and 3 females) out of 72 students antibody was moderate in 12.5%, and appropriate in
were not immune to this disease and there was no 75.9%. There was no significant relationship between
significant difference between males and females. Overall, antibody level and variables such as sex, age,
there was no significant difference between males and hospitalization ward, and body mass index of patients.
females in terms of antibody adequacy in the present study. Similar to the results of the present study, in 11.6% of the
This can be very dangerous considering the profession of subjects, the level of protective antibody was not
these students because they may be exposed to potentially measurable in their study, and therefore they recommended

103 Journal Dental School; Vol 39, No.3, Summer 2021; 102-105