Page 13 - Poster presentation KVAC 22
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                                                                            The 22 KhonKaen Veterinary Annual International Conference “Transboundary Animal Diseases (TADs)”











                                                                                                                 Transcutaneous Ligation of Vas Deferens








                                                                                                                                            for Preparation of Teaser Bucks










                                                                                                                                                                                                           1*
                                                                                                                                                                                                                                                                                                        2
                                                                                                                              Sarawut Sringam , Nittaya Boonbarn , Patchnee Sringam                                                                                                                                                                                                               3




                                                                               1  Unit of Theriogenology, Faculty of Veterinary Medicine, Khon Kaen University





                                                                               2  Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Khon Kaen University.




                                                                               3  Unit of Physiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand





                                                                               *  Corresponding author Email: sarsri@kku.ac.th











         Case Description                                                                                                                                                                                                                      After  surgery,  the  wounds  did  not  show  incidents  of




                                                                                                                                                                                                                                               swelling  or  edema.  The  libido  of  both  bucks  remained.
         Two  bucks,  one-year-old,  weighing  25  and  28  kg,  were


                                                                                                                                                                                                                                               Ultrasonography  was  performed  one  month  after  the

         subjected  to  study at  Veterinary Teaching  Hospital,  Khon




         Kaen University.  The  owner  wanted to  use  the  goats  as                                                                                                                                                                          surgery.  We  found  that  three  of  the  four  ligation  sites


                                                                                                                                                                                                                                               showed strictures of vas deferens. Only one from 4 ligation

         teasers.  Transcutaneous  ligation  of  vas  deferens  was


                                                                                                                                                                                                                                               sites  was  not  completed.  To  examine  the  semen

         performed on these bucks.


                                                                                                                                                                                                                                               ejaculation,  bucks  were  ejaculated  using  electro-





         Treatment and Outcome                                                                                                                                                                                                                 ejaculation  at  1,  3,  and  6  months  after  surgery.  Seminal






         The bucks were sedated with Xylazine. 0.02 mg/kg IV, the                                                                                                                                                                              plasma  from  the  bucks  were  clear  and  there  were  few




         animals  were  restrained  in  the  right  lateral  recumbent                                                                                                                                                                         sperms (total sperm <100,000 sperms) in their ejaculates,





         position. The scrotum was aseptically prepared, then was                                                                                                                                                                              the spermatozoa were non-motile.




         infiltrated with the 2.5 ml of 2% lidocaine HCl, at the level



                                                                                                                                                                                                                                                                                                                                                                          Vas deferen
         of  the  scrotal  neck  on  both  sides,  subcutaneously  and                                                                                                                                                                                                          Vas deferen





         distributed around the spermatic cords.                                                                                                                                                                                                                                        Testis











         Transcutaneous  ligation  was  achieved  by  thumbing  the





         spermatic cord at 3-5 cm above the pampiniform plexus to


                                                                                                                                                                                                                                               Figure 3. Ultrasound longitudinal image of vas deferens showing
         separate  vas  deferen far  away  from  blood  vessels.  Vas


                                                                                                                                                                                                                                               A) completed stricture (orange arrow) and B) dilatation (yellow

         deferen was pressed to beneath to skin; then the surgeon

                                                                                                                                                                                                                                               arrow) of vas deferens .

                                                                                                                         ®
         started  bite  suture  (Supramid 2/0)  through  the  skin,  in




         held vas deferens in the loop of the needle, after that the                                                                                                                                                                           Clinical Relevance




         needle  was  pulled  out  near  the  first  bite.  So  that  the                                                                                                                                                                      @ Teaser bucks are used for identifying does in estrus on





         suture  was  forming  a  complete  loop  around  the  vas                                                                                                                                                                             goat  farming.  Vasectomy  or  epididymectomy techniques





         deferens and the suture ends were tied ligating it.                                                                                                                                                                                   are  the  best  choices  for  sterilizing  the  buck  to  use  as  a





                                                                                                                                                                                                                                               teaser.  However,  those  techniques  require  aggressive




         The following ligation was done 3 cm above the previous                                                                                                                                                                               surgical intervention.





         ligation. The other side of vas deferens was done the same                                                                                                                                                                            @ Our study indicated that transcutaneous ligation of vas




         as  described  before.  The  bucks  received  antibiotics,  and                                                                                                                                                                       deferens was simply performed, with minor skin wounds,





         the sutures were removed ten days after surgery.                                                                                                                                                                                      and minimal postoperative care is needed.





                                                                                                                                                                                                                                               @However,  there  are  some  reports  in  human  about  the





                                                                                                                                                                                                                                               failure  of  vas  deferens  ligation  and  recanalization  of  vas





                                                                                                                                                                                                                                               deferens after surgery but incidence of failure is put at 0-




                                                                                                                                                                                                                                               3%.



                                                                                                                     Figure 1. The vas deferen (green                                                                                          @ For the confidence, the bucks should be conducted to




                                                                                                                     line) was separated from vessels
                                                                                                                                                                                                                                               examine the sperm before used them as teasers.


                                                                                                                     (red line) by thumbing before




                                                                                                                     biting the needle.                                                                                                       Keywords: Buck, Teaser, Vas deferens, Ligation










                                                                                                                                                                                                                                              References






                                                                                                                                                                                                                                              Adel  MB,  2009.  Percutaneous  ligation  of  spermatic  cord  as  an



                                                                                                                                                                                                                                              alternative to opened castration in donkeys. Benha Vet Med 20,





                                                                                                                                                                                                                                              24 -41.




                                                                                                                                                                                                                                              Amin T, Behrooz N, Masood S, Maryam M, Aboutorab TN, Saeed




                                                                                                                                                                                                                                              N, 2010. Epididymis ligation: a minimally invasive technique for




                                                                                                                                                                                                                                              preparation of teaser rams. Vet Surg 39, 121-127.




                                                                                                                                                                                                                                              Mellin HE,  Bauer  HW,  Rattenhuber U,  1980.  Failure  following



                                                                                                                  Figure 2. Each vas deferens was                                                                                             fertility vasectomy. Med Welt 31, 1723-1724.




                                                                                                                  transcutaneous                                                ligated                          (red                         Terry B, John C, 1996. Vasectomy in the ram. In Practice 18, 330-




                                                                                                                                                                                         ®
                                                                                                                  cross) with Supramid .                                                                                                      33.
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