Page 11 - Poster presentation KVAC 22

Molecular characterization of fowl aviadenovirus associated with inclusion

body hepatitis from infecting poultry based on partial hexon gene

1,4
2,4
2,4
Tawatchai Pohuang , Patchara Phuektes , Suphattra Jittimanee ,

3,4
1,4
Saikam Chaimongkol , Sucheeva Junnu *

1 Division of Livestock Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

2 Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand

3 Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

4
Research group for emerging and re-emerging infectious diseases in animals and zoonotic diseases Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, Thailand

*Corresponding author: sucheeva@kku.ac.th

Introduction Results and Discussions

• Fowl avidenoviruses (FAdV) are known to be TH/KKU01/2018IBH (MZ355711)

TH/KKU02/2018IBH (MZ355712)
associated with inclusion body hepatitis (IBH) [1] , 65 TH/KKU03/2018IBH (MZ355713)

causing significant economic losses to the poultry 77 TH/KKU04/2018IBH (MZ355714)

China FAdV-D SD15-24 (KY426992.1)

industry worldwide, including in Thailand. 31 South Korea FAdV-D ADL13 1851 (MN737052.1)

FAdV-D
Canada FAdV-D (MG832884.1)
• Its sudden onset and high mortalities characterize 8
92 Indonesia FAdV-D MSL/30/19 (MT104458.1)

the disease. 93 FAdV-2 (EU979368.1)

47 FAdV-11 (EU979378.1)

• FAdV has been subdivided into five species with 12 52 Thailand FAdV-D KU01/07 (EU678792.1)

FAdV-3 (EU979369.1)
serotypes based on their molecular structures and 99

92 FAdV-9 (EU979376.1)

[2]
cross-neutralization test results, respectively . FAdV-8a (EU979374.1)

FAdV-8b (EU979375.1) FAdV-E
80
• The hexon is the major capsid protein of FAdV and
76 FAdV-6 (EU979372.1)

contains genotypes-specific antigenic determinants 92 FAdV-7 (EU979373.1)

FAdV-5 (EU979371.1) FAdV-B

[3] and represents the most variable region for FAdV-1 (EU979367.1) FAdV-A

96 FAdV-4 (EU979370.1) FAdV-C
identifying and differentiating the 12 FAdV 100 FAdV-10 (EU979377.1)

[4]
serotypes .

0.05

• This study aimed to identify and characterize the Figure 1. Phylogenetic analysis of amino acid sequences of the hexon genes. Four

FAdV infecting poultry by using molecular isolates in the present study were marked with while other isolates were available
k

at GenBank with the accession number attached. The phylogenetic tree was

techniques based on the partial hexon gene. constructed using the neighbor-joining method with 1,000 bootstraps replicating on

Mega 6.

Materials and Methods • The four isolates of this study have 100% homology

with FAdV-D SD15-24 from China. With 95.8-100%

and 91.7-100% identical to the nucleotide and

• A total of 4 tissue samples have been

deduced AA sequences, respectively.

collected in 2018 from clinical cases

• Our strains were responsible for IBH outbreaks in

of FAdV infection of 4 broiler breeder

poultry in Canada [5] , China [6] , Indonesia [7] , and
farms in Thailand.

[8]
South Korea .
• Hexon gene of FAdV was detected

• Phylogenetic analyses revealed that clustered with

using PCR with a primer set:

FAdV-D; the FAdV-A, FAdV-B, FAdV-C, and FAdV-

HF: 5’-CGGGTCAGATGACAACTCCC-3’

E viruses, were more distantly related (Figure 1).

HR: 5’-CGGGATAGGTTAGCGAGGCG-3’

• The viruses may vertically transfer from the same

• The BankIt tool was used for the

grandparent flock to their progeny and spread

analysis of the sequence data

laterally among the flocks [10] .

submissions in the GenBank database.

TH/KKU01/IBH2018,

Conclusion

TH/KKU02/IBH2018,

TH/KKU03/IBH2018, and

In conclusion, phylogenetic analysis of the FAdV

TH/KKU04/IBH2018 have been
hexon gene of these sequences proved that they are

deposited in GenBank databases with
closely related to a strain previously classified as

the accession numbers MZ355711,

serotype FAdV-D.

MZ355712, MZ355713, and MZ355714,

respectively.

Acknowledgements
• The phylogenetic tree was constructed

using the neighbor-joining method by

The authors would like to thank the research group

bootstrap test 1000x. SUBMIT to GenBank

for emerging and re-emerging infectious diseases in

animals and zoonotic diseases, Faculty of Veterinary

Medicine and the Faculty of Veterinary Medicine,

Khon Kaen University, Khon Kaen University,

Thailand, for the grant support this project.

Sample of IBH lesion and sequence chromatogram of FAdV-D

QR-References