Page 12 - Poster presentation KVAC 22

nd
The 22 KhonKaen Veterinary Annual International Conference “Transboundary Animal Diseases (TADs)”

The Efficacy of Surgical Laparotomy Technique

for Artificial Insemination in Goats

2
3
1*
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Sarawut Sringam , Suvaluk Srisupa , Peerapat Deesuk , Avirut Wichaiwong , Patchanee Sringam 4
1 Unit of Theriogenology, Faculty of Veterinary Medicine, Khon Kaen University.

2 Unit of Surgery, Faculty of Veterinary Medicine, Khon Kaen University.

3 Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Khon Kaen University.

4 Unit of Physiology, Faculty of Veterinary Medicine, Khon Kaen University. Khon Kaen 40002, Thailand

Corresponding author: sarsri@kku.ac.th

Introduction

Artificial insemination (AI) is a powerful tool to improve livestock productivity in many species such as cattle, horses, pigs,

including goats. Although the efficacy of using semen frozen, thawed, and inseminated in many species was high, there

was limited in goat. To gets higher pregnancy rates with post-thaw semen, the spermatozoa should be deposited directly

into the uterus using laparoscopic artificial insemination (LAI). However, the disadvantage of LAI is that the LAI equipment

is expensive, and the procedures required well-trained veterinarians. In our study, we proposed the surgical laparoscopic

insemination technique (SLIT) that may be choice alternative for farm service. This study will show the success of SLIT in

terms of surgical time and conception rate.

Keywords: Surgical laparotomy, Artificial Insemination, Goats

Materials and methods

Estrus synchronization

Five does were estrus synchronized using an intravaginal progesterone device (Eazi-

breed™CIDR®; 0.3 g of medroxyprogesterone) for 12 days. On the day of CIDR removal, 300

IU pregnant mare serum gonadotropin (Folligon®) and 125 ug of cloprostenol (Estrumate®)

were intramuscular injections.

Surgical laparoscopic insemination technique

The insemination time was 54 hours after CIDR removal. All does were restricted from

water for 12 hours and feed for 24 hours before submitted to the operation. They were

intravenously sedated with 0.02 mg/kg of xylazine HCl (X-zine®). After 15 min, they were

intravenously anesthetized with 2 mg/kg of tiletamine-zolazepam (Zoletil®100). The does

were positioned in right lateral recumbency.

The surgical area was 3 cm projected from the udder to the inguinal region. The 5 cm skin

incision was extended. The subcutaneous tissues, muscle sheaths, and peritoneum were

carefully opened. The uterine horn was exposed by the index finger. A 24-gauze hypodermic

needle is used to inject the frozen-thawed semen (80 million motile spermatozoa) into the

lumen of the uterine horn. The peritoneum, muscle sheaths, and skin were separately

sutured. After surgery, all does received 2 mg/kg of flunixin (Finadyne®) intramuscular

injection for one day and 1.5 mg/kg intramuscular injection of cefquinome sulphate

(Cobactan®) for seven days. The operation time and the kidding rates were recorded.

Results and Discussions

Surgical area of all does were not showed edema or inflammation. The surgery was

15-25 (average=20.5) min in each doe. The conception rate and number of kids per

doe were 100% and 1.4 (7 babies from 5 does), respectively. Although LAI is the

minimally invasive surgical procedure and gives the high fertility rate (60-80%) (4) but

most veterinarian services did not have an expensive instrument. The time for

surgical LAI in this study was approximately 20.5 min compared to previous reports;

those were 5-15 min (4). There were no problems after surgery in this study. The

surgical LAI may be an alternative technique for keeping a higher success rate in goat

production.

Acknowledgment: The authors would like to thank the Faculty of Veterinary Medicine, Khon Kaen University, for the operation facility

and the nice cooperation from our fifth year students.

References

Rodriquez F, et al. 1988. Cervical versus intrauterine insemination of ewes using fresh or frozen semen diluted with aloe vera gel. Theriogenology 30(5), 843-854.

Kulakksiiz R, Daskin A. 2012. Reproductive performance of primiparous and multiparous Saanen goats after laparoscopic intrauterine insemination: a field study. Turk J

Vet Anim Sci 36(2), 201-204.

Menchaca A, Rubianes E. 2007. Pregnancy rate obtained with short-term protocol for timed artificial insemination in goats. Reprod Dom Anim 42, 590–593.

Davila FS, Gonzalez AS, Barragan HB. 2018. Reproduction in Goat. Intech Open.87-104. [cited 2021 June 28]