Page 6 - Poster presentation KVAC 22

Risk factors associated with MRS in skin disease dogs

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Danudet Detwattanatam Tawinun Saengprasert Thidarat Srisuwan Jaruwan Kampa
1 Fifth year student, Faculty of Veterinary Medicine KhonKaen University

2 Pathobiology, Faculty of Veterinary Medicine KhonKaen University

Introduction Material and Methods

Resistance to beta-lactam of Staphylococci or

Bacterial

Methicillin-resistant Staphylococci (MRS) is a identification and

Antibiotics
CULTURE ON
crucial problem causing treatment failure in canine Swabs BLOOD AGAR suscceptibility

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pyoderma . As Staphylococci is the common cause by Vitek® 2

(BioMérieux inc.)

of pyoderma in dog especially Staphylococcus

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pseudintermedius . The study aimed to identify the

risk factors of MRS infection in the canine cases - Pet information

attended to Veterinary Teaching Animal Hospital, Questionnaire - Health concerns Chi-square test

- Antibiotic treatment history
Khon Kaen University.

Fig. 1 Staphylococci colony in sheep blood agar

Fig. 2 Pyoderma lesion of dog samples

Result

The 42 Staphylococci isolates were found in 41 dogs, of which 18 isolates (42.8%) were MRS. The 13 MRS isolates

(30.9%) were Methicillin-resistant Staphylococcus pseudintermedius (MRSP) and were Multi-drug resistance (MDR).

MRS were found in dog that received systemic antibiotic more than 2 times in the past 12 months with significant

differences at p<0.05 .

S. sciuri

S.haemolyticus MRS Nitrofurant

Aminoglyc
MSR 2.4% oside, 12% oin, 2.4%

2.4% Beta-

MRS S. schleiferi MRS Trimethopr lactam,

im-sulfa,
42.8 4.8% 21% 43%

MRSA Fluoroquin

MSS 2.4% MRSP olone, 26% Clindamyci

n,
57.2% 30.9% 33%

Erythromy Doxycyclin

cin, 30% e,

Fig. 3 Prevalence of MRS 30%

Fig. 5 Prevalence of antibiotics resistance in MRS
Conclusion Fig. 4 Prevalence of MRSP, MRSA and others MRS

The repeated uses of systemic antibiotics was a significant risk factor of MRS in canine pyoderma. Regarding of the

high prevalence of the MRS, especially MDR, every canine pyoderma case should be testing with antibiotic

susceptibility test for an appropriate treatment plans.

Reference:

1 Bryan, J., Frank, L.A., Rohrbach, B.W., Burgette, L.J., Cain, C.L., Bemis, D.A. 2012. Treatment outcome of dogs with methicillin-resistant and methicillin-susceptible Staphylococcus pseudintermedius

pyoderma. Vet Dermatol. 23(4), 361–e65.

2 Bannoehr, J., Ben Zakour, N.L., Waller, A.S., Guardabassi, L., Thoday, K.L., van den Broek, A.H., Fitzgerald, J.R. (2007). Population genetic structure of the Staphylococcus intermedius group: insights

into agr diversification and the emergence of methicillin-resistant strains. J Bacteriol. 189(23), 8685–8692.