Page 4 - Poster presentation KVAC 22

Prevalence and Antimicrobial Resistance of Salmonella Isolated from

Pork and Hospitalized Patients in Vientiane Capital, Lao PDR

Dethaloun Meunsene , Pakpoom Tadee 2,*
1

1 Graduate Program in Veterinary Science, Faculty of Veterinary Medicine, Chiang Mai University, Muang, Chiang Mai, Thailand

2 Integrative Research Center for Veterinary Preventive Medicine, Faculty of Veterinary Medicine,

Chiang Mai University, Muang, Chiang Mai, Thailand

*Corresponding author: pakpoom.t@cmu.ac.th

Keywords: Antimicrobial resistance, Lao PDR, Patient, Pork, Salmonella

Introduction murium and S. Newport (Figure 1). Some of human salmonellosis in Vientiane Capital, Lao PDR

seems to be associated with pork consumption. However, it cannot be concluded that these types of

Non-typhoidal Salmonella (NTS) is denoted as a significant public health problem, samples are sharing a pool of Salmonella sources, exactly. Be better, genetic relatedness studies are

globally. Each year, 94 million infected cases with 155,000 deaths have been reported [1]. needed to be completed.

In Laos PDR, incidence of NTS Salmonella contaminated in food production chain (farm,

slaughterhouse, and retails) were reported at high level [2,3]. Although, there are very few Pork (%)

studies of human salmonellosis and the association of them with food animals’ origin. With S. London 32 (45.71)

the fact, pork is the most common type of meat consumed in this region [4]. Therefore, in S. Kedougou 8 (11.43) Patient (%)

the order to that knowledge gap, this study aimed to investigate the prevalence and S. Rissen 3 (4.28) S. Rissen 2 (16.66)

antimicrobial resistance of Salmonella in pork at retail markets and patient in hospital at S. Corvallis 4 (5.71) S. Kedougou 1 (8.33)

Vientiane Capital, Laos PDR. Understanding of their characteristics will inform the local S. Give 4 (5.71) Shared serotypes S. Hvittingfoss 1 (8.33)

epidemiology in transmission dynamics and provide the basis for applicable treatment S. Meleagridis 3 (4.28) S. Kedougou S. Typhimurium 1 (8.33)

options to help control the spread of salmonellosis in the area. S. Hvittingfoss 2 (2.85) S. Newport 1 (8.33)

S. Typhimurium 2 (2.85) S. Rissen S. Agona 1 (8.33)

Materials and Methods S. Altona 2 (2.85) S. Hvittingfoss S. Bareilly 1 (8.33)

S. Amsterdam 2 (2.85) S. Typhimurium S. Eingedi 1 (8.33)

S. Anatum 2 (2.85) S. Newport S. Stanley 1 (8.33)

S. Havana 2 (2.85) S. Uganda 1 (8.33)

S. Newport 1 (1.42) S. Weltevreden 1 (8.33)

Location & S. Brimingham 1 (1.42)

Source S. Goma 1 (1.42)
S. Planckendael 1 (1.42)

Vientiane capital Hospitalized Patient 5 Targeted marketplaces

Lao PDR Pork 1 Hospital Figure 1. Salmonella serotype isolates from pork in retail markets and patient at hospital at Vientiane capital

Lao PDR

We measured the susceptibility of the 82 isolates to 10 different antimicrobials by disk diffusion test.

In this study, both of CIP and NOR were able to inhibit all 82 of Salmonella isolates. 34.29 %

(24/70) of pork isolates and 75 % (9/12) of patient isolates were categorized as multidrug resistant

Sample collection strains. Resistance in pork isolates to TE was showed the highest rate (75%), followed by the AMP
Sample collection 93 of pork & 250 of gastro- (44%), SXT (44%), respectively. Likewise, considering in patients isolates, the resistance of AMP

intestinal tract disorders Individually Packing &

associated-hospitalized label Refrigerated (83%), TE (75%) and SXT (66%) were also detected in high level (Figure 2). Thus, closed to the

patients storage study of Boonmar et, al. (2013) [2] and Le Thi Phoung et al. (2017) [7]. Salmonella is often resistant

to broad spectrum antimicrobials widely used in human and veterinary medicine. However, based on

the evidence currently available, our findings indicate that the fluoroquinolones are still required for

salmonellosis treatment.

90
AMR

80

70

Isolation and All detected Serotyping was 60

identification were isolates used the serum- 50

performed following agglutination test (%) 40

ISO6579: 2002
Laboratory Amendment 1:2007, according to the 30

Procedure Annex D technique. White-Kauffmann- 20
Le Minor scheme

[5] 10

0

AMP AMC C CIP CTX NA NOR S SXT TE MDR

Antimicrobial susceptibility test by disk diffusion method [6], including: Antibiotic agents

Amoxicillin-clavulanic acid (AMC) 20/10 µg, Ampicillin (AMP) 10 µg,

Chloramphenicol (C) 30 µg, Ciprofloxacin (CIP) 5 µg, Cefotaxime (CTX) Pork Patient

30 µg, Nalidixic acid (NA) 30 µg, Norfloxacin (NOR) 10 µg,

Streptomycin (S) 10 µg, Sulfamethoxazole-trimethoprim (SXT) Figure 2. Antimicrobial resistance proportion of Salmonella strains from pork and patient in Vientiane capital,

23.75/1.25 µg & Tetracycline (TE) 30 µg Lao PDR.

Results and Discussion Acknowledgement

The authors would like to thank the Bacteriology Section, Veterinary Research and Development Center (Upper

Northern Region), Lampang, Thailand and Department of Veterinary Medicine, Faculty of Agriculture, National

A total of 343 samples, 93 from pork and 250 from the patients were included. Salmonella University of Laos for their valuable contributions. We also thank the Faculty of Veterinary Medicine, Chiang Mai

was detected in 75.27% (70/93; 95% CI= 65.24-83.63%) and 4.8% (12/250; 95% CI= 2.5- University for the grant support the graduate project.

8.23%), respectively. The previously prevalence reports of pork in retail market at southern

part of Laos was found at 93% (25/27; 95% CI= 75.71-99.09%) [2] that was higher than our References

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