Page 18 - §顯微鏡
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(iii)肝後黃疸:阻塞性黃疸
檢查結果:Blood: Conjugated bilirubin ↑
Urine: Blirubin(+) , UBG↓
Feces: 顏色較淡
(3)檢查方法:
- Bilirubin
(I)Yellow foam
(ii)Diazo reaction: Bilirubin 與 diazonium salt 作用生 light brown to pink
(iii)Oxidation test: Bilirubin → Biliverdin
- Gmelin test:定性測驗
- Harrison test
- Smith's iodine test
(iv)Confirmatory test: Ictotest 因能濃縮膽紅素,故敏感度較紙條法更高。
F(+):含色素的尿液檢體
F(-): Ascorbic acid, Light exposure (Nitrite 過高時,會減弱反應)
-Urobilinogen
臨床意義:肝臟疾病的早期偵測
溶血性疾病、紫質尿症
檢查方法:
(1)Reagent strip:Ehrlich's reagent(p-dimethylaminobenzaldehyde)
(2)Watson-Schwartz test:分別加入 Butanol 與 Chloroform
UBG:可溶於 Butanol 與 Chloroform
PBG: 不溶於 Butanol 與 Chloroform
Ehrlich-reactive substances: 只溶於 Butanol
(3)Hoesch test: PBG only
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