Page 13 - Live-cellanalysis handbook
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Cell Culture Quality Control Assays
Figure 3 shows results of a study to assess optimal levels of fetal bovine serum (FBS) and
cell density using the IncuCyte system.
Live-cell analysis can be used to accurately mask different morphologies and quantify
label-free proliferation over time, as shown in Figure 4 below. In 4A and 4B, the variation
in cell seeding density-dependent proliferation in two different cell lines, A172 human
glioblastoma and LNCap human prostate carcinoma, is exemplified. Understanding
cell seeding variation and its effects on proliferation is important for controlling assay
variability. As shown in 4C, images reveal accurate segmentation of cell types irrespective
of morphology.
Figure 3. Human umbilical vein endothelial cells (HUVECs) cultured in basal cell media containing growth factors and supplemented with
decreasing amounts of FBS. The optimal concentration of FBS for normal propagation was determined to be >2.5%.
A C
B
Figure 4. Quantification of A172 human glioblastoma (A) and LNCap human prostate carcinoma (B) growth curves using label-free confluence
analysis. (C) HD-phase contrast images of A549 human lung carcinoma, HT-1080 human fibrosarcoma and LNCap cells shown without and with
confluence mask (orange). Images reveal accurate confluence masking of all three cell types of varied morphology.
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