Page 14 - Live-cellanalysis handbook
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Live-Cell Analysis Handbook — Third Edition
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Figure 5. Images (A) and data (B) illustrating the effect of pipetting errors on growth. Wells were seeded at the same cell density and then checked prior to
an endpoint assay for growth. Initial confluence for four wells were found to be ~50% that of the median initial confluence for the plate, a variable likely
caused by pipetting errors.
Conclusions
With real-time live-cell analysis it is possible to follow and
quantify cell growth over time, effectively revealing both
transient and time-dependent phenomena. This type of analysis
is a powerful tool for cell culture quality control, providing a
quantitative, objective, non-invasive and kinetic method of
analyzing living cells, unperturbed in the incubator. Use of the
IncuCyte system to document and monitor routine cell culture
can improve cell-based assay quality and consistency.
Figure 6. T-25 flask exhibiting uneven growth, often related to poor
culture technique. Two representative images from a single flask show
differences in confluence.
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