Page 17 - Live-cellanalysis handbook

Kinetic Cell Health and Viability Assays

Greenberg, et. al., studied whether Bcl-2–IP3R interaction is a potentially useful therapeutic target in small cell lung carcinoma (SCLC). The
IncuCyte platform was used to detect caspase activation and nuclear condensation in SCLC cell lines.
Synergistic killing of human small cell lung cancer cells by the Bcl-2-inositol 1,4,5-trisphosphate receptor disruptor BIRD-2 and the BH3-mimetic
ABT-263. Greenberg, E. F., McColl, K. S., Zhong, F., Wildey, G., Dowlati, A., Distelhorst, C. W. Cell Death and Disease 2015 6:e2034.

Yang, et. al., describe characterization of a quadruplex dye (G4-REP; quadruplex-specific red-edge probe) that provides fluorescence
responses regardless of the excitation wavelength and modality thus allowing for diverse applications and most imaging facilities. Authors
demonstrated use via cell images and associated quantifications collected through use of the IncuCyte real-time live-cell imaging system
over an extended period, monitoring both non-cancerous and cancerous human cell lines.
Real-time and quantitative fluorescent live-cell imaging with quadruplex-specific red-edge probe (G4-REP). Yang, S. Y., Amor, S., Laguerre, A.,
Wong, J. M. Y., Monchaud, D. Biochimica et Biophysica Acta (BBA) - General Subjects http://dx.doi.org/10.1016/j.bbagen.2016.11.046.

Ano, et. al. sought to elucidate specific compounds that strongly suppress microglial inflammation by screening dairy products
fermented with Penicillium candidum. Quantitative live cell imaging to assess apoptosis or neuronal cell-death was performed using an
IncuCyte real-time imaging system. Images were obtained every 3 hour at 20x magnification in phase contrast mode. The number of
cells that had undergone apoptosis or cell-death was determined using the object counting algorithm.
Identification of a novel dehydroergosterol enhancing microglial anti-inflammato ry activity in a dairy product Fermented with Penicillium
candidum. Ano, Y., Kutsukaka, T., Hoshi, A., Yoshida,, A., Nakayama, H. PLoS On 10, e0116598, 201.

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