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Şekil 2: TNO gastro intestinal model cihaz ve şematik görüntüsü
Barsak epitelinin kültüre edilerek kullanıldığı in vitro
modeller
Gastro intestinal sistemin in vitro yapay sindirim sistemi ile
değerlendirmeleri sırasında farklı fazlarda alınan örneklerin
hücresel düzeyde değerlendirmelerinin yapılması mümkündür.
Bu kapsamda çok sayıda farklı hücre dizileri kullanılmaktadır.
İnsan kolon Ca hücreleri olan Caco-2 hücreleri en sık
kullanılan barsak epitel modelidir. Bunun yanısıra sistemin
çok güçlü mukozal immunitesinin varlığı besin maddelerinin
yarattığı immunojenik yanıtın değerlendirilmesini ve olası alerjik
reaksiyonların değerlendirilmesini zorunlu kılmaktadır. Bu
kapsamda da insandan elde edilmiş farklı lenfositik hücre serileri
doğrudan veya farklılaştırılarak kullanılabilmektedir.
KAYNAKLAR
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