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The Effect of Chlorogenic Acid in Robusta Lampung Green coffee extract (Coffee Canephora
Var Robusta) as an antioxidant in layer chicken infected with Salmonella enteritidis bacteria

Lilik Eka RADIATI , Djalal ROSYIDI , Dahliatul QOSIMAH , Indah Amalia AMRI and Dodik PRASETYO
1*
1
2
3
2

1 Animal Product Technology Laboratory, Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia
65145
2 Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang,
Indonesia 65151
3 Animal Clinical, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia 65151

( * Corresponding author’s e-mail: lilik.eka@ub.ac.id)

Abstract
The poultry industry has an essential role in fulfilling population nutritional needs around the world. S.
enteritidis bacteria infection causes high mortality and low poultry production. The rate of mortality and illness are
connected to gastrointestinal diseases and caused by suboptimal absorption. Coffee contains a high level of
Chlorogenic Acid (CGA), an antioxidant and anti-inflammatory compound. This research used layer chicken ISA
brown day old chicken (DOC). Treatments were divided into five groups with 12 replications: C+ group (chicken
infected by S.enteritidis 10 8 CFU/ml), C- group (healthy chicken), T1 group (chicken infected by S.enteritidis 10 8
CFU/ml and coffee extract 500 mg/kg BW (bodyweight)), T2 group (chicken infected by S.enteritidis 10 8 CFU/ml and
coffee extract 1000 mg/kg BW), T3 group (chicken infected by S.enteritidis 10 8 CFU/ml and coffee extract 1500
mg/kg BW). The Nrf2, HO-1, and SOD levels were measured by BD cell quest ProT programmed through the
flowcytometry method. Data of total cells were calculated for their average value and calculated by One Way ANOVA
statistical test (p<0,5). This research showed that giving coffee ethanol extract 500 mg/kg BW dosage could
increase Nrf2 and SOD level but decrease HO level. This research showed that Lampung robusta coffee extract
could potentially be an anti-inflammatory compound and antioxidant.

Keywords: poultry, zoonosis bacteria, coffee, antioxidant, free radical

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