Page 37 - Abstract Book ICOH4

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The Effect of Ketamine-Xylazine and Ketamine-Diazepam Combination on Erythrocyte
Morphology and Differential Leukocyte of Lovebird

Tiara Widyaputri*, Nurul Fauziah, Nofan Rickyawan, Dian Vidiastuti
Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia
Corresponding author: widyaputritiara@ub.ac.id

ABSTRACT

Anesthesia is the act of eliminating pain in the body from activities that cause pain. The anesthetic agent
that is usually used is the injection of a combination of ketamine-xylazine and ketamine-diazepam anesthetic
agents. The purpose of this study was to determine the effect of a combination of ketamine-xylazine and ketamine-
diazepam anesthetic agents on erythrocyte morphology and differential leukocyte in lovebird. This study was
conducted with 18 lovebirds which were divided into two groups, namely group A which will was given ketamine-
xylazine anesthesia with each dose of 50 mg/kg and 10 mg/kg and group B which was given ketamine-diazepam
anesthesia with each dose of 30 mg/kg and 2 mg/kg. The volume of anesthesia was given by injection into the
pectoral muscle. Blood sampling were taken through the brachial vein and then carried out a blood smear on the
object glass. Observation of erythrocyte morphology and leukocyte differential was carried out using an optilab
microscope with 1000x magnification. Erythrocyte morphological data were described descriptively and the
differential leukocyte were processed by the Wilcoxon Signed Rank Test. The morphology of erythrocytes in both
ketamine-xylazine and ketamine-diazepam showed a slight change in the erythrocyte nucleus. The use of ketamine-
xylazine and ketamine-diazepam showed no significant changes to the lovebird leukocyte differential.

Keywords: anesthesia, erythrocyte, leucocyte, lovebird.

TH
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