Page 34 - Abstract Book ICOH4

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Antibacterial activity test of Lampung Robusta coffee (Coffea canephora Lampung) extract
against Pathogenic Bacteria in Fish by In vitro

Dahliatul, Q., Djalal, R , Lilik, E. R., Indah, A.A., Dodik, P , Agri, K.A, Maftuch, Andi, J, Oktarina,
2
.
6
6
3
. 4
1
2
5
S, Indra, S.P
6

1 Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia,

2 Laboratory of Animal Product Technology, Faculty of Animal Husbandry, Brawijaya University, Indonesia
3 Animal Clinic, Faculty of Veterinary Medicine, Brawijaya University, Indonesia
4 Laboratory of Pharmacology, Faculty of Veterinary Medicine, Brawijaya University, Indonesia
5 Aquaculture Study Program, Faculty of Fisheries and Marine Sciences, Brawijaya University, Indonesia
6 Fish Quarantine Center, Fisheries Product Quality and Safety Control Surabaya I, Surabaya, Indonesia

*Corresponding Author E-mail: dahliatulqosimah@gmail.com/dahlia_qosimah@ub.ac.id

ABSTRACT :

Antibacterial using herbs has been done but using Lampung Robusta coffee up to now there is no scientific
evidence. Coffee contains active substances chlorogenic acid, tannins and alkaloids which can kill bacteria by
damaging the membrane and cell wall of the bacteria so that the bacteria lysis. Experimental research using robusta
green coffee bean extract Lampung consisting of five concentrations are: 0.1 g/ml; 0.2 gr/ml; 0.3 gr/ml, 0.4 gr/ml
and 0.5 gr/ml. Bacterial isolates used Streptococcus iniae, Pseudmonas aguilliseptica, Aeromonas salmonicida
and Yersinia ruckeri. Bacterial inhibition testing uses the Diffusion Disk method by measuring the diameter of the
inhibition zone, and the MIC (Minimum inhibition Concentration) method followed by the MBC (Minimum Bactericidal
Concentration) method. Results showed that that the coffee extract at all concentrations have inhibitory to bacteria
Streptococcus iniae, and Aeromonas salmonicida by the formation of inhibition zone diameter, whereas the MIC
value is obtained in Pseudomonas inguillitica. Coffee extracts with concentrations ranging from 0.3 g / ml showed
inhibitory properties against Yersinia ruckeri. Coffee extract cannot kill all test bacteria. The conclusion of the
research is that Robusta Lampung coffee extract has the potential to be bacteriostatic rather than bactericidal.

Keywords: Lampung coffee, MIC, bacterial pathogens, bacteriostatic

THE 4 INTERNATIONAL CONFERENCE ON ONE HEALTH (ICOH)
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“Strengthening Collaboration in One Health Systems”