Page 39 - Abstract Book ICOH4

28

Acute Toxicity Study of The Yogurt Fortified with Ethanol Extract of Roselle Calyces Modified the
Mice Gastric Inflammation
Aldila Noviatri , Delsa Nataya Honessy Saragih , Ani Setianingrum
3
1,*
2
1,2,3 Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, East Java, 65151, Indonesia

*Correspondence: noviatria25@ub.ac.id

ABSTRACT
Roselle (Hibiscus sabdariffa L.) has been used as a medicinal plant throughout the world. It contains flavonoids
that have pharmacological activities. Yogurt fortification with rosella calyces extracts increased the antioxidant
content of the yogurt. Consumption of high amounts of antioxidants needs to be evaluated for safety aspects.
This study aims to determine the effect of acute administration of fortified yogurt with roselle calyces ethanol
extract on the inflammation of mice (Mus musculus) gastric. Twenty mice were divided into four groups: the
control group (fed with standard diet) and three test groups were given single oral administration of yogurt fortified
with ethanol extract of roselle calyces at doses 1000, 1400, and 7000 mg/kg body weight. They have been
observed for 14 days and euthanized on the 15 day. The inflammation in the gastric of mice is known based on
th
the measurement of MDA levels using the TBARS method and the scores of chronic inflammation cell and
neutrophil infiltration on gastric histopathology. There was no defect in signs, animal behavior, and health
observed in the test groups compared to the control group. The result indicated that the MDA levels of the test
groups were significantly different (p<0.05) compared to the control group. Scoring on gastric histopathology
illustrated a significant rising (p<0.05) in chronic inflammation cell and neutrophil infiltration in mice that received
yogurt fortified with 7000 mg/kg dose of roselle extract compared to the others. Overall, our study demonstrated
that the single oral administration yogurt fortified with doses 1000, 1400, and 7000 mg/kg bodyweight of roselle
extract does not cause acute toxicity but escalates the MDA level, and inflammation cell scores the mice gastric.

Keywords: inflammation; MDA; neutrophil; roselle extract; yogurt

THE 4 INTERNATIONAL CONFERENCE ON ONE HEALTH (ICOH)
TH

“Strengthening Collaboration in One Health Systems”