Page 35 - Abstract Book ICOH4

24

Histomorphometry Characteristics of Jejunum Mus musculus Induced by Probiotics and
Salmonella sp.

Sruti Listra Adrenalin , Dini Agusti Paramanandi , Andreas Bandang Hardian , Hasna Izzatushsholihah
2
1*
3
Habiebillah , Nindy Afrillia Yulardianto Hamzah
4
4
1 Laboratory of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas
Brawijaya, Indonesia,
2 Laboratory of Veterinary Histology, Faculty of Veterinary Medicine, Universitas Brawijaya, Indonesia,
3 Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Brawijaya, Indonesia,
4 Student of Faculty Veterinary Medicine, Universitas Brawijaya, Indonesia

*Correspondence: srutilistra@ub.ac.id

ABSTRACT
Salmonellosis is a disease caused by Salmonella sp., which can cause infections of the digestive tract. Probiotics
are good microorganisms that can benefit the host because they can increase the normal flora of bacteria in the
digestive tract. They can maintain the intestinal mucosal barrier and prevent bacterial adhesion. The purpose of this
study was to determine the histomorphometric characteristics of the jejunum from the intestines of mice (Mus
musculus) after being infected with Salmonella sp. A total of 20 mice aged 4-6 weeks were divided into four research
groups: P1 (probiotics and Salmonella sp. infection), P2 (probiotic administration), P3 (Salmonella infection only),
and P4 (control). The probiotics used contain microorganisms such as Lactobacillus casei, Saccharomyces
cerevisiae, and Rhodopseudomonas palustris, dissolved in distilled water in a ratio of 1:1000. Probiotics were given
8
orally for 7 days. Salmonella sp. infection was given orally, with a volume of 0.5 ml (1.5 x 10 CFU/ml). The results
showed that the mean score of intestinal lesions was different between groups. The width of the villi, the thickness
of the mucosa, and the depth of the intestinal crypts were significantly different. The best histological picture was
in the group of mice that induced with probiotics only.

Keywords: Histomorphometry, jejunum, lactic acid bacteria, probiotics, Salmonella sp.

TH
THE 4 INTERNATIONAL CONFERENCE ON ONE HEALTH (ICOH)

“Strengthening Collaboration in One Health Systems”