Page 36 - Abstract Book ICOH4

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Red Ginger (Zingiber officinale var. rubrum): Its Immunostimulatory Potential on Broiler
Chickens Infected with Salmonella enteritidis

Agri Kaltaria Anisa 1*†, Herawati 2†, Jola Rahmahani 3†, Rahaju Ernawati 3†, Fedik Abdul Rantam 3†
1Laboratory of Veterinary Pharmacology, 2 Laboratory of Veterinary Public Health
Faculty of Veterinary Medicine, Universitas Brawijaya
Puncak Dieng Ekslusif, Kalisongo, Kec. Dau, Kab. Malang, East Java, Indonesia 65151
3Laboratory of Virology and Immunology, Department of Microbiology
Faculty of Veterinary Medicine, Universitas Airlangga
Mulyorejo Kampus C Unair Surabaya, East Java, Indonesia 60115

*Corresponding author: agri_kaltariaa@ub.ac.id
ABSTRACT

Salmonella enteritidis (S. enteritidis) is one of many bacterial species still found to infect chickens in
Indonesian farms. Due to its limited effectiveness and contribution towards resistance, the World Organisation of
Animal Health no longer recommends antimicrobials as therapy for Salmonella infection. Thus, plants with
immunostimulatory effects have been used as alternatives to increase chicken immune capacity to prevent and
fight Salmonella infections. This study proposed to assess the potential capability of Red Ginger (Zingiber officinale
var. rubrum) to boost the cellular and humoral arms of the chicken immune system.
A total of 20 1-day-old CP707 broiler chickens were divided into four treatment groups (n=5). We did not
subject group 1 to any treatments. We infected group 2 with 1x10 7 CFU/ml Salmonella enteritidis. While groups 3
and 4 we supplemented with 2% red ginger powder and 13.5 mg/kg BW Stimuno ® forte, respectively, in addition to
being infected with S. enteritidis. Serum was separated from blood samples taken on days 1, 6, 13 and 20 post-
infection. The Enzyme-Linked Immunosorbent Assay (ELISA) was used to measure the serum concentrations of
Interferon-γ (IFN-γ) and Immunoglobulin A (IgA).
This study implies that 2% red ginger does not potentially increase the cellular immune response of
chickens infected with S. enteritidis, as it cannot significantly increase the serum concentration of IFN-γ (P>0,05) at
any given analysis time. Meanwhile, 2% red ginger demonstrated a potential immunostimulant effect on the humoral
immune system, as shown by the significant increase of IgA (P<0,05) serum concentrations on days 1 and 13 post-
infection. However, red ginger's immunostimulatory potential is lower than the commercially available immune-
stimulating product Stimuno ® forte.

Keywords: broiler chickens, IgA, IFN-γ, immunostimulant, red ginger

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