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290 Materials and Nanotechnology | Progress Report
with globular structures (Figure
3), typical of the apatite, indicat-
ing the bioactivity of the studied
composites.
The osteoblast adhesion tests
with MG63 line cells showed the
positive effect of the vitreous
phase on the bioactivity, since the
number of cells increased as the
culture time for all studied sam-
ples. The bone formation ability
of the samples can also be veri-
fied by the large areas of calcified
matrix shown in the image of the
surface stained with alizarin after
14 days of cell culture (Figure 4).
Hence, considering the results of
Figure 3: Image of a silicon nitride sample submitted to bio-
activity test in SBF after 16 days of immersion densification, microstructure, me-
chanical properties and in vitro
ceramics with conventional compositions. biological behavior, the obtained
silicon nitride composites is a promising for
Backscattered scanning electron micrographs applications in biomedical devices, mainly in
of the samples surfaces submitted to SBF tests intervertebral disc spacers, artificial l disc re-
indicate the formation of a superficial layer placement and acetabular components in to-
Figure 4: Light microscopy
image of alizarin red S staining
for mineralization in MG-63
cells for a silicon nitride sample
after 14 days of culture.
Instituto de Pesquisas Energéticas e Nucleares
