Page 129 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 40 Page 1 of 1

40. Estimation of Hydrogen Peroxide Assay

2
Expected H O 2
SCOPE
Suggested sample amount (gms)
concentration (wt. %)
To estimate the assay of Hydrogen peroxide. 0.25 - 1.0 100 - 250
1 - 3 30 - 100
APPARATUS
FAnalytical Balance, 1 mg / 0.1 mg accuracy 3 - 10 10 - 30
FVolumetric Flask, 250 ml 10 - 35 3 - 10

FPipette, 25 ml, Burette, 10 ml 35 - 70 1.5 - 3.0
FBeaker, 500 ml
FWash the weighed sample into 250ml volumetric
flask with distilled water, dilute to the mark and mix
REAGENTS
F0.3N Potassium permanganate thoroughly.

FConcentrated Sulphuric acid. FPipette a 25ml aliquot into a 500ml beaker
containing 250ml distilled water and add 10ml of
con.sulphuric acid. Titrate to the first permanent
PROCEDURE
pink colour with 0.3N KMnO .
FFrom the table below, transfer an appropriate 4
aliquot of Hydrogen peroxide sample to a tare
weighing bottle and weigh on an analytical balance.

CALCULATION

T.V X N.of KMnO X 0.01701 X 1000
4
% of H O =----------------------------------------------------------
2 2
Sample Weight, g

Prepared by : Approved by :
117 Director - Technical