Page 126 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 37 Page 1 of 5

37. Estimation of Choline Chloride Assay

4. Add 10 ml mercuric acetate solution. (5%)
PRINCIPLE
Choline in an acidic solvent(acetic acid) behave as a 5. Add 2 drops of crystal violet indicator.
base which when titrate against per chloric acid , under
6. Titrate the solution with 0.1 N per chloric acid in
goes a neutralization reaction whose end point can be
determined by using crystal- violet indicator . Mercuric burette
acid is used to remove the interference of Halide ion as 7. Note the color change from violet to blue and finally
Mercuric halide. to green.

8. Run the blank without taking sample by repeating
PROCEDURE
the steps 2 to 7.
1. Take 300 mg choline in 250 ml conical flask.
Note: Take 5 g of Mercuric acetate in a 100 ml SMF
2. Add 50 ml Glacial Acetic Acid and warm on
steam bath until choline chloride is dissolved and make up to the mark by using Glacialacetic acid
completely. giving 5% solution.

3. Cool the solution to room temperature.

CALCULATION
Vs - Vb X 0.01396 X 100
Choline chloride (%) = ------------------------------------------
Wt of sample, g

Where,
Vs - Is the volume of per chloric acid in ml required by sample.

Vb - Is the volume of per chloric acid in ml required by blank
Molecular weight of choline chloride is139.6

Prepared by : Approved by :
114 Director - Technical