Page 121 - Feed Lab

Laboratory Water Suguna Management System Ver 1.0 / SOP / FQL / P2 - 33 Page 2 of 3

STANDARD CURVE
Blank S1 S2 S3 S4 S5 S6
STD.UREA (ml) - 0.1 0.2 0.3 0.4 0.5 0.6
BUFFER (ml) 5 4.9 4.8 4.7 4.6 4.5 4.4
P-DMAB (ml) 5 5.0 5.0 5.0 5.0 5.0 5.0
UREA conc.(mg/ml)- 0.1 0.2 0.3 0.4 0.5 0.6

FMix the content in the test tube using cyclo mixer
Picture - 7 Picture - 8
and let it stand for 10 minutes in Refrigerator.
REAGENTS (Picture - 9)

FP-DMAB Solution: Dissolve 1. 6g of p - Dimethyl FMeasure OD of the Std. Urea against Blank using
Amino Benzaldehyde in 100ml of Alcohol and 10ml Colorimeter at 420 nm. A plot of OD vs. Urea
concentrated hydrochloric acid. It is stable for one concentration should be straight line. (Picture - 10)
o
week under low temperature below 24 C).

PROCEDURE
FZinc Acetate: Dissolve 22.0g of zinc acetate, LR
grade in water. Add 3ml of acetic acid and dilute to FWeigh 1gm ground sample into 100ml volumetric
100ml. flask. Add 1gm Charcoal, about 50ml distilled
water, 5ml Zinc acetate solution and 5ml
FVegetable Charcoal Powder G-60 Potassium ferrocyanide solution. Shake for
30minutes and dilute to volume with water. (Picture
FPhosphate Buffer Solution: (pH - 7.00): Dissolve
- 11)
0 . 3 4 0 3 3 g o f a n h y d r o u s P o t a s s i u m
dihydrogenortho phosphate, LR grade and FAfter the precipitate settle down, decant the
0.4355g anhydrous dipotassium hydrogen supernatant through the filter paper. Collect the
phosphate, LR grade separately in 10 ml portion of filtrate, pipette 5ml of filtrate into a test tube and 5ml
distilled water. Mix the two solutions and dilute to p-DMAB solution. (Picture - 12)
100ml distilled water.
FShake it well and stand for 10minutes in
FStandard urea solution: Take 0.5g urea AR grade refrigerator. Read OD at 420nm against a reagent
is dissolved in distilled water and make up to blank of 5ml distilled water and 5ml of p-DMAB.
100ml. This solution contains 5mg urea per ml. (Picture - 13)

FPotassium Ferrocyanide Solution: Dissolve 10.6
gm Potassium ferrocyanide solution LR Grade in
water and made up to 100 ml.

Picture - 9 Picture - 10

Prepared by : Approved by :
109 Director - Technical