Page 117 - Feed Lab

Estimation of Unsaponifiable Matter Suguna Management System Ver 1.0 / SOP / FQL / P2 - 30 Page 2 of 2

FAvoid removing any of the petroleum ether layers. FMix the weighed residue in the alcohol solution and
Continue to wash with the 10% ethyl alcohol again titrate with 0.02 N NaOH till the end point.
solution until the wash solution no longer gives
a pink Colour after the addition of one drop of
phenolphthalein solution.
FTransfer the petroleum ether extract to tarred flat
bottom flask and evaporate to dryness on a soxhelt
heater.

FAfter all the solvent has evaporated, complete the
drying to constant weight in an oven at 120ºC ,
cool in a desiccators and weigh. The result
becomes "A" in the calculation.

FTake 50 ml of alcohol (95%) in a conical flask. Add
2-3 drops of Phenolphthalein indicator in to it and
titrate with 0.02 N NaOH till the neutralization.

CALCULATION

A - (B + C)
Unsaponifiable matter % = -------------------------------- x 100
Weight of sample (gm)

Where, A = Weight of residue
B = Weight of fatty acid,
C = Weight of blank.

REFERENCE : AOCS Ca6a-40

Prepared by : Approved by :
105 Director - Technical