Page 24 - Feed Lab

Lab Equipments Maintenance & Handling Procedure Suguna Management System Ver 1.0 / SOP / FQL / P1 - 02 Page 4 of 6

substances in contact with an oxidant are the receiver.
explosive.
FAllow sufficient time for drainage of liquid from the
FReactions requiring cylinders of toxic, flammable or pipette, and then touch the liquid with the tip of the
reactive gases should be run in fume hoods, and pipette. Small amount remaining in the pipette must
suitable racks should be provided to hold the not be blown since allowance for this small quantity
cylinders. is made while standardizing the pipette.

FIn case of fire, turn off the flammable gas, then Reading:
oxidizing gas if possible!
Colourless fluid -lower meniscus
FDo not extinguish a flame involving a highly
combustible gas until the source of gas has been Coloured fluid - upper meniscus
shut off. Otherwise it can re-ignite with an Caution: For transferring ammonia solution or
explosion. concentrated acids, use safety type of pipettes.

FRemove regulator from almost empty cylinder and Burettes: These measuring devices are designed to
replace protection cap at once; using tape or tag, deliver variable volumes of liquid. The burette should
label the cylinder as "EMPTY". be rinsed with the liquid to be used and then filled
slightly above the mark as a general rule it is advisable
FFor cylinders used in GC/HPLC analysis moisture
traps shall be used and checked regularly. to start from the zero mark each time; when using a
burette, it is necessary to record initial and final
readings. Both the initial and final reading should be
COMMON LABORATORY APPARATUS AND
taken by folding the burette at a height where
TECHNIQUES
graduation to be read is in level with the observer eye.
Conical flask (Erlenmeyer `s flask): Conical flasks of After use, clean the burettes and rinse with the distilled
200 - 500 ml capacity find many applications, for water.
example, in titration.

o
Funnels: Funnels should enclose an angle of 60 . The Reading:
most useful sizes for quantitative analysis or those with Colourless fluid - lower meniscus;
diameters of 5.5, 7 & 9 cm. The stem should have an
internal diameter of about 4 mm and should not be Coloured fluid - upper meniscus
more than 50 mm long. Measuring cylinders: Although these measuring
containers are not capable of delivering the exact
amounts, these are widely used in laboratory to
Pipettes:
FThese are used to measure and dispense a known measure the total volume of solutions or liquids with
volume of liquid. When using pipettes first clean lesser accuracy.
the outer well of the pipette with a clean tissue
paper, then rinse it out with a little of liquid to be Volumetric flask: Useful for preparing standard
measured. solutions, they have long narrow neck with one mark
around the neck.
FThen by suction fill the pipette, a little above the
mark, close with the finger and hold at a height Calibration of Volumetric Apparatus
where the graduation is level with your eyes. FFor most analytical purposes, volumetric apparatus
manufactured to class 'A' standard will prove to be
FThen allow the liquid to run down until the lower
satisfactory, but for work for the highest accuracy it
meniscus is at the graduation level. Touch the tip of
pipette to bottom of the glass vessel and hold it in is advisable to calibrate all apparatus for which is a
an upright position, allow the liquid to discharge into recent test certificate is unavailable.

Prepared by : Approved by :
14 Director - Technical