Page 25 - Feed Lab

Lab Equipments Maintenance & Handling Procedure Suguna Management System Ver 1.0 / SOP / FQL / P1 - 02 Page 5 of 6

FThe calibration procedure involves determination of resistant protective gloves when inserting glass into
the weight of the water contained in or delivered by a stopper or tubing; insert the glass so that the
the particular piece of the apparatus. applied force is away from the body.

FThe temperature of the water is observed, and from FDo not use excessive force.
the known density of water at that of temperature
the volume of water can be calculated. FDo not stopper glass flask containing hot
condensable vapours.
FTables giving density values are usually based on
weights in vacuum, but the data given in table are FDispose of chipped or broken glassware in the
based on weighing in air with stainless weights, and Broken Glass receptacle. All broken glassware
must be immediately swept up and removed from
these can be used to calculate relevant value
directly from the observed weight of water. the bench or floor and placed in a receptacle
provided solely for that purpose.
FIt is suggested that the data given in the table be
plotted on a graph so that the volume of 1g of water FDo not place any glassware in general waste
at the exact temperature at which the calibration containers.
was performed can be ascertained. FNotify your supervisor of any significant breakage.

FBroken glassware may be placed in a rigid, sealed
Volume of 1g of water at various temperatures:
container (e.g. a box). Label the container "Broken
O O
Temp. ( C) Volume (ml) Temp. ( C) Volume (ml) Glass."
12.00 1.0015 24.00 1.0037
14.00 1.0017 26.00 1.0044 Sharps:

16.00 1.0021 28.00 1.0047 FTake care not to stick yourself with a needle.
18.00 1.0023 30.00 1.0053 FDo not place used syringes in pans containing
pipettes or other glassware that require sorting.
FIn all calibration operations, the apparatus to be FNeedles, blades, etc. are considered hazardous
calibrated must be carefully cleaned and allowed to even if they are sterile, capped and in the original
stand adjacent to the balance, which is to be container.
employed, together with distilled or de-ionized
water so that it assumes the temperature of the Fume Hoods
room. FHoods should be turned off and the sash closed
when not in use. If the hood is a variable speed
FFlask will also need to be dried, and rinsing twice
hood it cannot be turned off, just make sure the
with a little acetone and then blowing a current of air
through the flask to remove the acetone can sash is closed when not in use.
accomplish this. FEquipment and other materials should be placed
and all work should be performed a minimum of 15
cm (6 inches) behind the sash opening. This
Attention:
FWear heat resistant gloves or use tongs when practice will reduce exposure of laboratory area to
handling glassware or equipment that has been chemical fumes due to air turbulence.
heated.
FWhen the hood is in use, the sash should be kept at
FWhen glassware is ready to by cleaned, take it to the optimum air flow height. A sticker affixed directly
the wash area, and specify any special cleaning next to the sash indicates this height. The sash is
instructions. your primary barrier for protection against fire and
explosion that may take place in fume hoods.
FLubricate all contact surfaces and wear cut/slash

Prepared by : Approved by :
15 Director - Technical