Page 70 - Feed Lab

Estimation of Ether Extract Suguna Management System Ver 1.0 / SOP / FQL / P2 - 05 Page 2 of 2

FDisconnect the receiving flat bottom flask, wipe the
outside of the flat bottom flask thoroughly with a
clean dry cloth to remove the moisture, dust and
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dry it is a hot air oven at 105 C+/-20 C for 2 hours.
If any ether is left over, it will be evaporated out.
(Picture - 11)

F Cool the flat bottom flask in desiccators and weigh.
(Picture - 12)
Picture - 5 Picture - 6

CHEMICALS
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Petroleum ether, 60 C - 80 C
PROCEDURE
FTake around 2-3 gm of ground sample (Weigh the
sample in the ordinary filter paper and fold it) into the
thimble. Put a cotton plug into the thimble over that
thimble. Take a clean dry receiving flat bottom flask Picture - 7 Picture - 8
of 250ml capacity and weigh accurately.
(Picture - 7)

FIntroduce that thimble with sample into the Soxhelt.
The top of the thimble should be well below the
level of the siphon bend. Put the flat bottom flask in
the heating mantle and fill it with 150ml to 175ml
petroleum ether. (Picture - 8)

FAssemble the Soxhlet with flat bottom flask. The
Picture - 9 Picture - 10
temperature in the heating mantle should be
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between 80 C and 90 C. Please note that cold
water should circulate through the condenser.
Extract continuously for 4 hours. (Picture - 9)

FAfter extraction is over, remove the thimble with
sample inside from the Soxhelt. Assemble the
apparatus again and reheat to recover all ether from
the receiving flat bottom flask. The flat bottom flask
now contains only the crude fat. (Picture - 10)
Picture - 11 Picture - 12

CALCULATION
( Weight of dry fat flat bottom flask) - (weight of dry empty flat bottom flask)
Ether Extract % = ---------------------------------------------------------------------------------------------- X 100
Sample weight, g

REFERENCE
1. Bureau of Indian Standard IS 7874 1975
2. Association of Official Analytical Chemist 2000.

Prepared by : Approved by :
58 Director - Technical