Page 71 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 06 Page 1 of 2

6. Estimation of Crude Fibre

SCOPE
To estimate the Crude Fibre in feed and feed stuffs.

PRINCIPLE
FWhen a sample of moisture and fat free plant
material is boiled first with dilute acid and then with
dilute alkali, the soluble carbohydrates and proteins
go into solution and thus removed.

FThe undissolved residue left behind represents Picture - 1 Picture - 2
cellulose hemi cellulose and lignin. All these put
together are called Crude Fibre.

FWhen this residue after drying and weighing is
heated in an oven and a shed in a muffle furnace,
the fibre being organic in nature is burnt away
leaving behind the ash which represents mainly the
mineral matter. The fiber content is obtained by
deducting the weight of ash from the weight of the
dried residue before putting furnace.

Picture - 3 Picture - 4
Apparatus
F Analytical Balance, 1 mg / 0.1mgaccuracy
(Picture-1)
F Hot Air Oven (Picture-2)
F Muffle furnace (Picture-3)
F Hot plate (Picture-4)
F Tall form beaker without spout, 500 ml (Picture-5)
FFilter flat bottom flask (Picture-6)
FMuslin cloth - more than 18 threads per cm
(Picture-7)
F 250 ml Flat bottom flask as condenser (Picture-8) Picture - 5 Picture - 6

Prepared by : Approved by :
59 Director - Technical