Page 109 - Live-cellanalysis handbook
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Kinetic Neurite Analysis Assays
Neurite length–fluorescent neurotrack
Neurite length Neurite length
(mm/mm ) 2 (mm/mm )
2
200 200
150 150
100 100
50 50
0 0
0 100 200 300 400 0 100 200 300 400
Time (h) Time (h)
Kainate 1 mM Kainate 1 mM + NBQX and MK801
Kainate 0.33 mM Kainate 0.33 mM + NBQX and MK801
Kainate 0.11 mM Kainate 0.11 mM + NBQX and MK801
Kainate 0.01 mM Kainate 0.04 mM + NBQX and MK801
Kainate 0.04 mM Kainate 0.01 mM + NBQX and MK801
Kainate 4.12e-3 mM Kainate 4.12e-3 mM + NBQX and MK801
Control Control
Cell viability–IncuCyte Annexin V NIR
®
NIR object NIR object
confluence (%) confluence (%)
8 8
6 6
4 4
2 2
0 0
0 12 24 36 48 60 72 0 12 24 36 48 60 72
Time (h) Time (h)
Figure 7. Kainate exposure concentration- and time-dependently decreases neurite length and increases cell death in iGluta Neuron co-culture. Time course
of the effects of kainate addition at day 14 (arrow) on neurite length (top panels) and cell viability (bottom panels). Kainate causes a concentration-
dependent decrease in neurite length with concomitant decrease in cell viability (increased IncuCyte Annexin V NIR fluorescence). Addition of NBQX (20
μM) and MK801 (10 μM) protects iGluta Neurons from kainate toxicity. Each data point represents mean +/- SEM, n=6.
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