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                                                                  a

                                                                                              b
          Table 3.3  Pairwise per cent amino acid sequence identity based on fusion  and hemaagglutinin-neuraminidase  proteins and evolutionary
                         c
          divergence estimate  based on fusion gene coding sequences of avian paramyxovirus serotypes
          APMV serotype  APMV-1   APMV-2   APMV-3   APMV-4   APMV-5   APMV-6   APMV-7   APMV-8   APMV-9   APMV-10
          APMV-1         *        40.8     31.4     32.1     39.5     38.7     37.4     41.7     55.3     40.2
                                  35.2     34.9     34.7     34.7     31.0     36.2     34.5     61.7     34.6
                                  0.926    1.072    1.135    0.954    1.023    1.042    0.965    0.603    0.948
          APMV-2                  *        30.6     33.5     47.2     50.0     38.5     63.4     38.5     61.0
                                           31.5     32.1     42.7     41.3     42.4     48.3     32.9     51.1
                                           1.173    1.107    0.823    0.823    0.902    0.572    0.975    0.615
          APMV-3                           *        32.4     30.4     32.1     27.1     31.9     28.3     29.7
                                                    40.1     32.4     31.6     33.9     31.3     36.5     32.0
                                                    1.041    1.126    1.117    1.207    1.073    1.129    1.182
          APMV-4                                    *        33.2     33.3     29.9     36.0     28.8     33.7
                                                             30.7     32.3     36.3     32.7     35.6     30.8
                                                             1.034    1.018    1.151    1.045    1.143    1.058
          APMV-5                                             *        54.0     38.4     46.2     35.9     47.4
                                                                      56.3     42.6     41.2     32.4     41.7
                                                                      0.634    0.915    0.808    0.982    0.776
          APMV-6                                                      *        38.4     49.5     38.8     50.2
                                                                               43.6     39.8     31.2     40.8
                                                                               0.919    0.822    0.991    0.775
          APMV-7                                                               *        38.6     35.2     38.5
                                                                                        41.7     35.3     40.7
                                                                                        0.857    1.096    0.842
          APMV-8                                                                        *        38.7     60.8
                                                                                                 34.5     50.3
                                                                                                 0.936    0.607
          APMV-9                                                                                 *        39.7
                                                                                                          34.8
                                                                                                          0.957
          APMV-10                                                                                         *


          APMV-11

          APMV-12


          APMV-13

          APMV-14


          APMV-15


          APMV-16

          APMV-17


          APMV-18

          APMV-19


          APMV-20

          APMV-21 d

          a      b
           Green and  blue values indicate pairwise per cent amino acid sequence identities of fusion and haemagglutinin-neuraminidase proteins of APMV serotypes,
          respectively. The F and HN gene sequences used were taken from the genome sequences of respective APMV prototype strains; GenBank accession numbers cited
          in the Fig. 3.8. The amino acid per cent identity was calculated using ClustalW alignment of protein sequences in MegAlign program of Lasergene software. Per cent
          identity values from 50.0 and above are underlined.
          c
           Black values indicate pairwise evolutionary divergence estimates based on the complete coding sequences of fusion gene of avian paramyxovirus serotypes. The
          number of base substitutions per site between sequences are shown. Standard error estimate(s) ranged from 0.026 to 0.068. All ambiguous positions were removed for
          each sequence pair. Evolutionary analyses were conducted using the Kimura 2-parameter model (Kimura, 1980) in MEGA7 (Kumar et al., 2016).
          d Putative APMV serotype awaiting official recognition from the ICTV.
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