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98  |  Paldurai and Samal

                                                      a
                                                                                    b
          Table 3.4  Pairwise per cent nucleotide sequence identity  and evolutionary divergence estimate  based on genome sequences of avian
          paramyxovirus serotypes
          APMV serotype  APMV-1  APMV-2   APMV-3   APMV-4    APMV-5   APMV-6   APMV-7   APMV-8   APMV-9   APMV-10
          APMV-1        *        45.6     42.5     42.7      44.5     42.9     44.1     44.9     57.8     44.5
                                 0.974    1.105    1.110     1.019    1.096    1.036    0.992    0.621    1.022
          APMV-2                 *        43.2     43.7      48.7     46.8     49.1     57.6     44.6     60.4
                                          1.096    1.069     0.878    0.947    0.865    0.643    1.015    0.571
          APMV-3                          *        46.1      41.7     41.8     42.6     43.1     42.3     42.7
                                                   0.973     1.155    1.176    1.110    1.105    1.107    1.109
          APMV-4                                   *         42.6     41.6     42.8     42.4     42.2     42.5
                                                             1.106    1.154    1.099    1.123    1.117    1.092
          APMV-5                                             *        55.0     48.3     48.6     43.4     48.9
                                                                      0.701    0.888    0.877    1.058    0.865
          APMV-6                                                      *        46.7     45.7     42.2     46.6
                                                                               0.962    0.988    1.122    0.943
          APMV-7                                                               *        49.6     43.7     49.2
                                                                                        0.854    1.049    0.856
          APMV-8                                                                        *        45.1     58.2
                                                                                                 0.997    0.637
          APMV-9                                                                                 *        44.4
                                                                                                          1.013
          APMV-10                                                                                         *

          APMV-11

          APMV-12
          APMV-13


          APMV-14
          APMV-15

          APMV-16

          APMV-17

          APMV-18

          APMV-19

          APMV-20

          APMV-21 c

          a Green values indicate pairwise per cent nucleotide sequence identities calculated between genome sequences of APMV serotypes. See
          phylogenetic tree (Fig. 3.7) for the GenBank accession numbers of the genome sequences used. The nucleotide per cent identity was calculated
          using ClustalW alignment in MegAlign program of Lasergene software. Per cent identity values from 50.0 and above are underlined.
          b Black values indicate pairwise evolutionary divergence estimates between genome sequences of APMV serotypes. The number of base
          substitutions per site between sequences are shown. Standard error estimate(s) ranged from 0.007 to 0.025. Evolutionary analyses were
          conducted using the Kimura 2-parameter model (Kimura, 1980) in MEGA7 (Kumar et al., 2016).
          c Putative APMV serotype awaiting official recognition from the ICTV.
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