Page 32 - MNU-PM502- Pharmaeutical Microbiology Theoritical Book

Pharm D- Clinical Pharmacy Program Third Level Pharmaceutical Microbiology& Antimicrobials (PM 502)

Recommended temperature and duration

Temperature (℃) Pressure (psi) Time (min)
115 ℃ 10 psi 30 min
121 ℃ 15 psi 15 min
132 ℃ 27 psi 10 min

134 ℃ 30 psi 2 min

Types of Autoclaves
A. Based on size

1. Large-scale autoclave: It is more significant in size, can
have 500-1500 liters chamber.

• It is suitable for hospitals and clinical and research
laboratories.

2. Small-scale autoclave: It is smaller in size. It has chambers
that can simultaneously fit 20-300 liters of autoclavable

materials.

• It is suitable for university and college laboratories.

B. Based on principle

1. Gravity displacement autoclave: It is generally of two types;
horizontal and vertical autoclave.

• Horizontal autoclave: The door/lid of this type of autoclave open

outwards towards the handler. It is usually available in large
sizes.

• Vertical autoclave: The autoclavable material is loaded from the top
side of the autoclave. It is usually available in small sizes.

2. Pre-vacuum (prevac) autoclaves: This type of autoclave uses a

vacuum pump to remove all the air from the autoclave’s chamber,
allowing for better steam penetration.

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