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2.9.2.3 High-level disinfection
Definition: is a Process that eliminates all microorganisms except some bacterial
endospores from inanimate objects.
2.9.2.4 Chemical disinfection
Definition: This is the process of disinfecting used equipment by using chlorine/
Glutaraldehyde/ formaldehyde or peroxide.
Purpose: to eliminate microorganisms from inanimate objects.
Equipment
1. Chlorine, Glutaraldehyde, formaldehyde, and peroxide.
2. Container for disinfection
3. Heavy-duty gloves
4. Sterile containers
5. pick up forceps
Procedure
1. Wash hands and dry them
2. Prepare necessary equipment
3. Prepare fresh sterilant as per manufactures instructions
4. Submerge cleaned and dried items in 2% Glutaraldehyde (cidex) for 8 – 10 hours 80%
formaldehyde solution – 24 hours
5. Ensure items are completely immersed
6. Remove items from the chemical solution using sterile gloves, Forceps/pickups.
7. Rinse thoroughly with sterile water to remove all traces of chemical sterile
8. Use item immediately or store in sterile containers?
9. Wash hands and dry them.
2.9.2.5 Boiling
Definition: Boiling in water is an effective practical way to the high-level disinfectant
instrument and other items
Purpose
To kill all vegetative forms of bacteria, viruses (including HBV, HCV, and HIV)
Equipment
Water
Boiler
Stove
Sterile forceps
Sterile container ( high level disinfected container
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