Page 35 - Live-cellanalysis handbook
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Kinetic Caspase-3/7 and Annexin Apoptosis Assays


           Multiplex Assays to confirm apoptotic pathways
           The IncuCyte system can be used to detect and confirm apoptosis
           through two different pathways by multiplexing caspase-3/7 and
           annexin V reagents to verify apoptosis as a mechanism of cell death.



                 Caspase-3/7                        Annexin V                            Label-free confluence








































           Figure 6: Detect both caspase-3/7 and annexin V signals in cultures. Automatically determine the time courses of apoptotic cell death and correlate
           with label free confluence measurements to provide an estimate of the proportion of apoptotic cells within the population
           (apoptotic index).






























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