Page 106 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 23 Page 1 of 2

23. Estimation of Peroxide Value in

Animal Protein Sources

SCOPE
To estimate the Peroxide Value (PV) in Meat and Bone
meal and Fishmeal.

PRINCIPLE
Lipids undergo auto oxidation in presence of
atmospheric oxygen and metal ions to from peroxides
and hydro peroxide. Thus leading to oxidative rancidity. Picture - 1 Picture - 2
A known volume of the fat, oil or any given sample is
dissolved in Acetic acid - Chloroform mixture and
saturated potassium iodide is added in acidic medium.
The liberated iodine is titrated against standard sodium
thiosulphate using starch indicator. The liberated
iodine forms a blue colour complex, which on
titration becomes colorless. Further calculating gives
Peroxide Value, thus showing the degree of rancidity.

Picture - 3 Picture - 4
APPARATUS
FAnalytical Balance, 1 mg / 0.1 mg accuracy
(Picture-1)

FBurette, 10 ml (Picture-2)

FIodine flask, 250 ml, 500 ml (Picture-3)

FGraduated cylinder, 50 ml (Picture-4)

FGraduated pipette, 1 ml, 5 ml, 10 ml (Picture-5)
Picture - 5

Prepared by : Approved by :
94 Director - Technical