Page 102 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 21 Page 1 of 2

21. Estimation of Free Fatty Acid in

Animal Protein Sources

SCOPE
To estimate the free fatty acid (FFA) in Meat and Bone
meal and Fishmeal.

PRINCIPLE
Most lipids undergo hydrolysis liberating free fatty acid
resulting in hydrolytic rancidity. A known amount of the
Picture-1 Picture-2
sample is dissolved in ethyl alcohol and titrated against
standard sodium hydroxide solution using
Phenolphthalein as indicator. The result gives the
extent of hydrolytic rancidity that has taken place in
given sample.

APPARATUS
FAnalytical Balance, 1 mg / 0.1 mg accuracy
(Picture-1)
Picture-3 Picture-4
FErlenmeyer flask, 250 ml (Picture-2)

FGraduated Cylinder, 25 ml (picture-3)

FGraduated pipette, 10 ml (picture-4)

FBurette, 10 ml (picture-5)

Picture-5

Prepared by : Approved by :
90 Director - Technical