Page 100 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 20 Page 1 of 2

20. Estimation of Free Fatty

Acid in Oil and Tallow

SCOPE
To estimate the free fatty acid (FFA) in oil and tallow.

PRINCIPLE
Most lipids undergo hydrolysis liberating free fatty acid
resulting in hydrolytic rancidity. A known amount of the
sample is dissolved in ethyl alcohol and titrated against
standard sodium hydroxide solution using Picture-3 Picture-4
Phenolphthalein as indicator. The result gives the
extent of hydrolytic rancidity that has taken place in
given sample.

Apparatus
FAnalytical Balance, 1 mg / 0.1 mg accuracy
(Picture-1)
FErlenmeyer flask, 250 ml (Picture-2)

FGraduated Cylinder, 25 ml (Picture-3)
Picture-5
FGraduated pipette, 10 ml (Picture-4)
FBurette, 10 ml (Picture-5)
REAGENTS
F0.1N Sodium Hydroxide: 4gm of sodium
hydroxide AR grade in one litre of water.

FPhenolphthalein indicator.

FAbsolute alcohol.

Picture-1 Picture-2

Prepared by : Approved by :
88 Director - Technical