Page 98 - Feed Lab

Estimation of Multitoxin Suguna Management System Ver 1.0 / SOP / FQL / P2 - 19 Page 2 of 3

Picture - 5 Picture - 6 Picture - 13 Picture - 14

Picture - 7 Picture - 8 Picture - 15

FAcetonitrile, chloroform (redistilled / AR grade).

FSodium sulphate (activated)
F20% Sulphuric acid v/v, AR grade

Preparation of activated TLC plate
To prepare 2 plates (10cm x 10cm or 10cm x 5cm) of
0.2mm thickness dissolve 16g of silica gel (G) in 35ml
Picture - 9 Picture - 10
of water, apply on the plates using applicators and
0
allow it to natural dry. Then keep the plates at 105 c for
1 hour and cool. Draw lines with 1cm space such that
standards are at the middle of the plate and four sample
spots can be applied on either side of the standard.

PROCEDURE
FTake 10 gm of sample in a beaker. Add 36 ml of
Acetonitrile, 4 ml of 4% Kcl solution & 0.8 ml of 5N
HCl. Stir well this mixture for 5 mins & filter the

Picture - 11 Picture - 12 solution through filter paper. ( Picture - 16)
FCollect 20 ml of Filtrate, 20 ml of Distilled water & 20
REAGENTS
ml of Hexane in a separating funnel shake
F4% Potassium chloride in 5N Hydrochloric acid: vigorously. Collect the lower layer in the 2nd
20g of Potassium chloride is dissolved in 291ml of Separating funnel. Then add 20 ml of Hexane in the
distilled water. Add slowly 209 ml of hydrochloric
2nd funnel Shake vigorously. (Picture - 17)
acid in the above solution and shake well.
FCollect the lower layer in 3rd separating funnel. In

Prepared by : Approved by :
86 Director - Technical