Page 96 - Feed Lab

Estimation of Aflatoxin Modified Romer's Method Suguna Management System Ver 1.0 / SOP / FQL / P2 - 18 Page 4 of 4

spectrophotometer (vide AOAC 1990 15 the edition
PREPARATION OF AFLATOXIN STANDARD:
Ch: 49 pp 1185-1186). From this stock solution,
Carefully prepare the aflatoxin in a suitable standard prepare aflatoxin solution in benzene: acetonitrile
flask with a ratio of benzene : acetonitrile (98+2) mixture containing 4 g/ml in a suitable standard flask which is
to give a concentration of 10 g/ml. Standardize the the working standard.
concentration of the stock solution using

CALCULATION
S x C x D
Aflatoxin = ----------------------------------- X 1000
T x 1.714

S = Standard which compares with the sample in fluorescent intensity
C = Concentration of the standard.
D = Dilution factor
T =Sample which compares with standard in fluorescent intensity.

1.714 = Effective weight.

Prepared by : Approved by :
84 Director - Technical