Page 97 - Feed Lab

Suguna Management System Ver 1.0 / SOP / FQL / P2 - 19 Page 1 of 3

19. Estimation of Multitoxin (Modified Romer's Method)

FGlass micro syringe, 10 microlitre (picture - 10)
SCOPE
To estimate the multitoxin (Ochratoxin, Citrinin and T 2) FPre coated thin layer chromatography plate silica
in feed and feed stuffs by thin layer chromatography. gel (picture - 11)

FUV Cabinet (picture - 12)
PRINCIPLE
The multitoxin (Ochratoxin, Citrinin and T2) is extracted FDeveloping Tank (picture - 13)
with Acetonitrile and extract with oil by hexane. The
above extract is extracted with chloroform, dried, FHot Air Oven pre calibrated (picture - 14)
rediluted with Benzene Acetonitrile and spotted in an FHot plate or Water bath (picture - 15)
activated thin layer chromatography plate with
standards and ascertained the concentration by visual
comparison method in Ultra Violet 365 nm viewing
cabinet.

APPARATUS
FAnalytical Balance, 10 mg / 1 mg accuracy (picture
- 1)

FBeaker, 500 ml, 250 ml, 50 ml, 25 ml (picture - 2)

FLaboratory stirrer motorized (picture - 3) Picture - 1 Picture - 2
FGraduated cylinder, 100 ml, 50 ml, 10 ml (picture -
4)

FSeparating funnel, 250 ml, 100 ml (picture - 5)

FGlass funnel, 50 mm, 70 mm, diameter (picture - 6)
FFilter paper fluted (picture - 7)

FCulture tube, 5 ml (picture - 8)

FGraduated pipette, 1 ml, 5 ml (picture - 9) Picture - 3 Picture - 4

Prepared by : Approved by :
85 Director - Technical