Page 99 - Feed Lab

Estimation of Multitoxin Suguna Management System Ver 1.0 / SOP / FQL / P2 - 19 Page 3 of 3

3rd funnel, add 10 ml of Chloroform & shake 2 DEVELOPING
nd
vigorously. To collect the lower layer drop by drop
Take the Toluene, Ethyl Acetate & Formic Acid in the
through Sodium Sulphate Bed. (Picture - 18)
FAgain add 10 ml of Chloroform & Shake vigorously.
To collect the lower layer drop by drop through
Sodium Sulphate Bed. Dry the solution in water
bath & then spot. (Picture - 19)

SPOTTING
Add 0.2 ml of Chloroform in the dried beaker & cover
with aluminum Foil. Shake it gently. From this, take 40
micro litre the soln. & then spot in the TLC Plate's
corner. (Plate size should be 10 X 10 cm).
(Picture - 20)

st
1 DEVELOPING
Take the Chloroform, Acetone in the ratio of 9:1 (90ml
Chloroform + 10 ml Acetone) in a developing tank. Put
the spotted TLC plate in the developing tank. After
completion of developing, allow it to dry in the room
temp. (Picture - 21)

Picture - 16 Picture - 17 Picture - 18 Picture - 19

Picture - 20 Picture - 21 Picture - 22 Picture - 23

CALCULATION
Comparison X Concentration X Dilution (0.2)
Ochratoxin = --------------------------------------------------------------- X 1000
Sample Volume (40) X Effective Weight (4.9)
Comparison X Concentration X Dilution (0.2)
Citrinin = ---------------------------------------------------------- X 1000
Sample Volume (40) X Effective Weight (4.9)

Prepared by : Approved by :
87 Director - Technical