Page 101 - Feed Lab

Estimation of Free Fatty Acid in Oil and Tallow Suguna Management System Ver 1.0 / SOP / FQL / P2 - 20 Page 2 of 2

appearance of the first permanent pink colour of the
PROCEDURE
same intensity as that of the neutralized alcohol that
FCompletely melt the oil sample (in case of mutton
persist for 30 seconds. Record the volume and
tallow) in a beaker on a water bath, filter while hot
calculate the Free Fatty Acid. (Picture 9)
sample through filter paper if necessary. (Picture-6)

FHomogenize the sample and weigh 5gm of the EQUIVALENT FACTOR
designated sample into Erlenmeyer flask. TYPE OF OIL FATTY ACID FACTOR
(Picture-7) Corn oil Oleic 28.24

FAdd 50 ml of ethyl alcohol previously
Sunflower oil Lauric 20.03
neutralized with 0.1N sodium hydroxide
Palm/Peanut /
using Phenolphthalein indicator. (Picture-8) M.Tallow Palmitic 25.64
Soya / Rice
FBoil the mixture and titrate the hot mixture with 0.1 bran oil Linoleic 28.04
N sodium hydroxide shaking vigorously to

Picture - 6 Picture - 6 Picture - 6 Picture - 6

CALCULATION

V X N X F
Free fatty acid % = ----------------------------------------------
Sample weight, g

Where,
V - Volume of sodium hydroxide

N - Normality of sodium hydroxide
F - Equivalent factor.

REFERENCE
Association of Official Analytical Chemist 2000.

Prepared by : Approved by :
89 Director - Technical