Page 428 - The Veterinary Laboratory and Field Manual 3rd Edition

Plate 17 (Figure 4.33b) Scanned array image
showing positive (yellow) and negative results
(black). Each spot shown in the array corresponds
to a specific fluorescent value, determined by the
strength of hybridization, in a semi-quantitative
manner. Photos: Dr Shayan Sharif and Dr Jennifer
Brisbin, University of Guelph, Canada.

Plate 18 (Figure 4.33b & c) Real-time PCR amplification curves and the melting
peaks. (B) Representative amplification curves for the quantification of viral DNA
by real-time PCR based on SYBR Green chemistry. The DNA sample was serially
10-fold diluted and each dilution has assayed in triplicate. High DNA copy number
samples (lower dilution show amplification curves early during the amplification and
the low copy number or negative and no template control samples (higher dilution)
show late or no amplification. (C) Melting peak analysis shows the specific amplifi-
cation. Depending on the sensitivity of the assay low DNA copy number samples
yield no peaks as there is no template control. Photos: (b and c) Dr M. Faizal Abdul-
Careem, University of Calgary, Canada.

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