Page 430 - The Veterinary Laboratory and Field Manual 3rd Edition

Plate 21 (Figure 4.36) Phylogenetic Tree of a total of over 180 partial σC gene sequences of avian reo-
virus. The analysis included sequences obtained from the GenBank. Vaccine strains are shown in a bold
red colour. The tree shows several clusters of avian reovirus and most of the sequences are far from the
sequences of the avian reovirus vaccines. This raises the question whether vaccine induced immunity is
protective against the disease induced by these diverse avian reo viral strains. Source: Victor Palomino-
Tapia, University of Calgary, Canada.

Plate 22a See also Figure 5.6 Blood smear from Plate 22b See also Figure 5.7 Poor quality bovine
a cow which later died following fever, haematuria blood smear stained with Giemsa 100× oil immer-
and weight loss over a period of several days. The sion. Note the presence of a large mononuclear cell.
animal had concurrent Babesia bovis and Theileria The red cells are irregular in shape and there is a lot
sp. infection following a spring peak in tick popula- of artefact. The presence of granules suggests that
tions, Pemagatshel, Eastern Bhutan. Giemsa 20× new stain should be prepared.
magnification. The darker pigmented areas in the
red blood cells indicate stages of the life cycles of
the two protozoal species. See also Chapter 3.

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