Page 434 - Essential Haematology

420 / Chapter 30 Pregnancy and neonatal haematology

(a)

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Figure 30.5 (a) Kleihauer test for

fetal red cells; a deeply eosin -
staining cell containing fetal
haemoglobin is seen at the
centre. Haemoglobin has been
eluted from the other red cells by
an incubation at acid pH and
these appear as colourless
ghosts. (b) Determination by fl ow
cytometry of the number of Rh D
10 -1 10 0 10 1 10 2 10 3 fetal cells in maternal blood using
fl uorescent - label of antibody to
Anti-D FITC
Rh D, the mother being Rhdd.
(b) (Courtesy of Dr W. Erber.)

Figure 30.6 Doppler ultrasono-

graphy of the circle of Willis in a
fetus. The cursor is placed over
the middle cerebral artery and an
increased blood velocity corre-
lates with anaemia. (From Kumar
S. and Regan F. (2005) BMJ 330 ,
1255 – 8, with permission.)