Page 632 - Atlas of Histology with Functional Correlations

The majority of cells that line the liver sinusoids (5) are endothelial cells (2)

with an attenuated cytoplasm and a small nucleus. To demonstrate the
phagocytic cells in the liver sinusoids (5), an animal was intravenously injected
with India ink. The phagocytic Kupffer cells (3, 7) ingest the carbon particles
from the ink, filling their cytoplasm with black deposits and becoming prominent
in the sinusoids (5) between the hepatic plates (6). Kupffer cells (3, 7) are large

cells with several processes and an irregular or stellate outline that protrudes into
the sinusoids (5). The nuclei of Kupffer cells (3, 7) are obscured by the ingested
carbon particles.

Visible on the periphery of the lobule is a section of the interlobular

septum (1) and a part of the bile duct (4) lined with cuboidal cells.

FIGURE 16.7 ■ Kupffer cells in a liver lobule (India ink preparation). Stain:
hematoxylin and eosin. High magnification.

FIGURE 16.8 | Glycogen Granules in Liver Cells

(Hepatocytes)

The cytoplasm of hepatocytes varies in appearance depending on nutritional
status. After a meal, liver hepatocytes (1) store glycogen in their cytoplasm.

With the periodic acid–Schiff stain, the glycogen granules (2, 4) in the
hepatocyte (1) cytoplasm stain bright red and exhibit an irregular distribution
within the cytoplasm.

Also visible in this illustration are hepatic sinusoids (3) and flattened

endothelial cells (5) in their lumina.

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